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Image Search Results
Journal: Cancer letters
Article Title: Tyrosine phosphorylation of HSC70 and its interaction with RFC mediates methotrexate resistance in murine L1210 leukemia cells
doi: 10.1016/j.canlet.2014.11.036
Figure Lengend Snippet: Colocalization of HSC70 and RFC in L1210/0 and L1210/DDP cells. (A) HSC70 expression, RFC expression, and overlay (merged) image of HSC70 and RFC channels (specific wave lengths of fluorescent light) in L1210/0 cells with or without MTX. (B) HSC70 expression, RFC expression, and overlay (merged) image of HSC70 and RFC channels in L1210/DDP cells with or without MTX. The yellow fluorescence represents the expression of HSC70 immuno-tagged with alexa fluor 546. The red fluorescence represents the expression of RFC immune-tagged with alexa fluor 647. The images were captured with confocal laser scanning microscopy at 60× objective, 1024 resolution and 2.1 optical zoom and turned to 8-bit color and analyzed for colocalization using JACoP plugin from image J. The images shown are representative of three independent experiments. (C) 2D scatter plot colocalization of L1210/0 cells and L1210/DDP cells with or without MTX. The yellow channel (Alexa Fluor 546) for HSC70 is graphed along the X-axis and the red channel for RFC (Alexa Fluor 647) is plotted on the Y-axis. The plots shown are a representation of three independent experiments. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: The fluorescent emissions were quantified using
Techniques: Expressing, Fluorescence, Confocal Laser Scanning Microscopy